|
KMID : 1134820080370040410
|
|
Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 4 p.410 ~ p.415
|
|
|
Antimutagenic and Cytotoxic Effects of Kochujang Extracts Added Deep Sea Water Salt and Sea Tangle
|
|
Ham Seung-Shi
Choi Hyun-Jin
Kim Soo-Hyun
Oh Hyun-Taek
Chung Mi-Ja
|
|
|
Abstract
|
|
|
This study was performed to observe the antimutagenic and cytotoxic activities of methanol extract of
kochujang added with sea tangle and deep sea water salts (SDK) and kochujang added with sea tangle (SK)
using the Ames test and SRB assay, respectively. The direct antimutagenic effect of SDK and SK methanol
extracts were examined by Ames test using Salmonella Typhimurium TA98 and TA100. In the Ames test,
methanol extract of SDK and SK alone did not exhibit mutagenicity and most of the samples showed high
antimutagenic effects against mutation induced by N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) and
4-nitroquinoline-1-oxide (4NQO). Methanol extract of SDK (200 μg/plate) showed approximately 71.4%
inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain; whereas 56.1% and 83.6%
inhibitions were observed on the mutagenensis induced by 4NQO and MNNG against TA100 strain. The
cytotoxic effects of SDK and SK increased with increasing sample concentration against human cervical
adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human breast adenocarcinoma (MCF-7),
human stomach adenocarcinoma (AGS), and human lung carcinoma (A549). The SDK at the concentration
of 1 mg/ml showed cytotoxicities of 61.5%, 61.3%, 51.4%, 57.9% and 77.7% against HeLa, Hep3B, MCF-7,
AGS and A549, respectively. In contrast 1 mg/ml treatment of SDK and SK methanol extract had only 2～38%
cytotoxicity on human transformed primary embryonal kidney cell (293).
|
|
|
KEYWORD
|
|
|
deep sea water kochujang, antimutagenic, cytotoxic activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|